Re-emergence of pertussis despite high vaccination coverage in western countries, results in increased risk for severe and even fatal pertussis among newborns. For this reason, late 2015 the Dutch Health Council (HC) advised to offer 3rd trimester pertussis vaccination to pregnant women. At the start of the maternal pertussis programme late 2019, the maternal Tdap was advised from 22w of gestation onwards. Preterms, accounting for 8% of newborns in the Netherlands, are at highest risk for severe pertussis leading to prolonged hospital and intensive care admissions and sometimes death. Recently, it has become evident that despite 3rd trimester vaccination, preterms remain at high risk because the vaccination is likely given too late for sufficient antibody transfer. For this vulnerable group 2nd trimester vaccination may offer better protection because of extended time for antibody transfer. To date, most countries recommend 3rd trimester vaccination to protect young, not yet (fully) vaccinated infants. Data from England show 91% effectiveness against infant pertussis after maternal Tetanus- diphtheria -acellular Pertussis (Tdap) vaccination in the 3rd trimester. Studies focussing on preterms and protection after maternal vaccination are scarce. Two observational studies reported on effectiveness and antibody levels in cord blood of 2nd trimester vaccination in term infants. While one study showed significantly higher antibody levels after 2nd trimester vaccination (13-25 gestational weeks; GW), another study showed decreased effectiveness of 2nd trimester (<27 GW) vaccination. Only one study concerned antibody transfer in preterms and reported higher antibody levels after 2nd (n=37) than after 3rd (n=48) trimester vaccination. Aiming to contribute to setting optimal vaccine strategy of maternal pertussis vaccination in the Netherlands and elsewhere and particular for the most vulnerable group of preterms, we propose a study that compares pertussis antibody levels in preterms and terms after 2nd trimester maternal vaccination. We can compare these to data we have on 3rd trimester Tdap in terms. In addition to adequate antibody levels, success of 2nd trimester vaccination depends on acceptance of this strategy by pregnant women and professionals. Our primary endpoint is IgG anti-pertussis toxin (Pt) antibody concentration in preterms and terms at 2m of age, Pt is considered the most relevant antibody for protection against clinical pertussis. Secondary endpoints are e.g. pertussis specific antibody concentrations in preterms and terms in cord blood and in women at delivery. Determinants of acceptance of 2nd trimester maternal vaccination are also a secondary endpoint. Antibody concentrations will be assessed in serum, using a fluorescent bead-based multiplex immunoassay, with required blood volume of minimal 100µl. For the survey on acceptance, we aim to have 4 groups of 100 women each, i.e. women who are pregnant for the 1st time, women who already gave birth and in both groups women with and without a known increased risk of preterm delivery. For the immunogenicity part, we aim to have at least 60 preterms and 60 terms, as this is, according to experts, the minimum number to enable good comparisons. Pregnant women will be offered 2nd trimester pertussis vaccination. Both among acceptors and non-acceptors acceptance of 2nd trimester vaccination will be assessed. Women are first asked to participate in the acceptance part after the 1st antenatal visit to a midwife or obstetrician. They fill in a questionnaire to assess behavioral determinants and beliefs that underlie acceptance of 2nd trimester maternal vaccination. Only after this consent, women will be asked to participate in the immunogenicity part. Hereby, women will receive Tdap after they have the 20w standard anomaly ultrasound scan (20-24 GW). Vaccinated women will be followed until delivery. All preterms and a random selection of 60 terms, all of vaccinated mothers, will be followed until 2m of age, i.e. just before start of the NIP. By including both women in primary and secondary antenatal care, we aim to enrich our study population with women who are at increased risk for preterm delivery, as these women are usually seen by an obstetrician. Data from our study will determine whether 2nd trimester Tdap leads to sufficient Pt antibodiy concentration in terms and preterms compared to 3rd trimester vaccination. Furthermore, we will have knowledge about obstacles for acceptance and can tailor information for all pregnant women to overcome these. Finally, given that in near future besides pertussis other maternal vaccines are likely to become available for prevention of severe disease in newborns (RSV, GBS), in particular in preterms, this study generates essential knowledge for future vaccine policy of maternal vaccines.

<p>Datasets used for the manuscript:&nbsp;<em>Long-term wastewater monitoring of SARS-CoV-2 viral loads and variants at the major international passenger hub Amsterdam Schiphol Airport: a valuable addition to COVID-19 surveillance</em></p> <p><em>pandemic_daily_passenger_counts.tsv</em>: An overview of daily passenger arrival&nbsp;counts at Amsterdam Schiphol Airport per continent of origin during the study period 16-02-2020 - 04-09-2022</p> <p><em>pre-pandemic_daily_passenger_averages.tsv:&nbsp;</em>An overview of mean daily passenger arrival counts at Amsterdam Schiphol Airport in the pre-pandemic period 2017-2019.</p> <p><em>viral_load_data.tsv:&nbsp;</em>Flow-corrected viral load (# particles per 24h) in samples taken at the wastewater treatment plant of Amsterdam Schiphol Airport.</p> <p><em>wastewater_variant_frequencies.tsv:&nbsp;</em>SARS-CoV-2 lineage estimates in samples&nbsp;taken at the wastewater treatment plant of Amsterdam Schiphol Airport, analyzed using whole-genome tiled amplicon sequencing.</p>

Geluidkaart 2016 voor het Nederlandse hoofdspoor in het kader van de Regeling omgevingslawaai. Met de geluidkaart voor het hoofdspoor (de gemiddelde geluidswaarde van alle nachtperioden, 23.00 tot 7.00 uur, Lnight) wordt invulling gegeven aan de eisen uit de Wet milieubeheer die voortvloeien uit de Europese Richtlijn Omgevingslawaai. Over de kaart De kaart en de gegevens zijn afkomstig en eigendom van het ministerie van Infrastructuur & Milieu. De geluidkaart is gemaakt in het kader van de EU geluidkartering en bevat geluidcontouren variërend tussen 50 en 65 dB in klassen van 5 db. Op basis van de EU-regelgeving zal de volgende geluidkartering gaan over het jaar 2021 en waarschijnlijk in 2022 klaar zijn en online verschijnen.

Deze dataset bevat de jaargemiddelde cijfers van het Nationaal Meetnet Radioactiviteit van het RIVM zoals in het kader van het EURATOM verdrag verzameld. De data is op diverse meetlocaties verspreid over Nederland gemeten.

Deze geluidskaart laat zien hoeveel geluid het verkeer op een snelweg maakte in 2016. De kaart geeft de gemiddelde geluidswaarde van alle nachtperioden (23.00 tot 7.00 uur) weer. Op de kaart staan alle snelwegen die worden beheerd door Rijkswaterstaat. Geluidskaarten van andere wegen en andere geluidsbronnen zijn te vinden bij provincies en een aantal gemeenten. Wettelijk moeten deze geluidbelastingskaarten eenmaal in de vijf jaar worden ontwikkeld.

Genexpressie in keratinocyten na blootstelling aan sensibiliserende of irriterende stoffen

Het Landelijk Meetnet Grondwaterkwaliteit (LMG) is opgebouwd tussen 1979 en 1984 en bestaat uit ongeveer 350 meetlocaties die zijn verspreid over heel Nederland. Er wordt bemonsterd in permanente putten die speciaal voor monitoringsdoeleinden zijn aangelegd. Deze waarnemingsputten zijn net buiten de velden aangelegd om eenvoudig te kunnen bemonsteren en de werkzaamheden in het veld niet te hinderen. De locaties zijn geselecteerd op basis van grondsoort, het landgebruik en de hydrologische toestand. Op elke locatie worden grondwatermonsters genomen op diepten van 5-15 m (ondiepe filters) en 15-30 m onder het maaiveld (diepe filters). Op zandgrond worden uit ondiepe waarnemingsputten elk jaar monsters genomen, terwijl er op de andere grondsoorten (klei en veen) elke twee jaar monsters worden genomen uit ondiepe putten. Uit diepe putten wordt elke vier jaar een monster genomen, evenals uit ondiepe filters op meetpunten met mariene invloeden. De putten die niet elk jaar worden bemonsterd, worden in geïnterpoleerd voor de afwezige jaren.

genexpressie in stamcellen na blootstelling aan ontwikkelingstoxische stoffen

Genexpressie in longen van C3H muizen na infectie met Bordetella pertussis

genexpressie van rattenembryos in vivo en in whole embryo culture; ontwikkeling en respons op retinoïnezuur