Re-emergence of pertussis despite high vaccination coverage in western countries, results in increased risk for severe and even fatal pertussis among newborns. For this reason, late 2015 the Dutch Health Council (HC) advised to offer 3rd trimester pertussis vaccination to pregnant women. At the start of the maternal pertussis programme late 2019, the maternal Tdap was advised from 22w of gestation onwards. Preterms, accounting for 8% of newborns in the Netherlands, are at highest risk for severe pertussis leading to prolonged hospital and intensive care admissions and sometimes death. Recently, it has become evident that despite 3rd trimester vaccination, preterms remain at high risk because the vaccination is likely given too late for sufficient antibody transfer. For this vulnerable group 2nd trimester vaccination may offer better protection because of extended time for antibody transfer. To date, most countries recommend 3rd trimester vaccination to protect young, not yet (fully) vaccinated infants. Data from England show 91% effectiveness against infant pertussis after maternal Tetanus- diphtheria -acellular Pertussis (Tdap) vaccination in the 3rd trimester. Studies focussing on preterms and protection after maternal vaccination are scarce. Two observational studies reported on effectiveness and antibody levels in cord blood of 2nd trimester vaccination in term infants. While one study showed significantly higher antibody levels after 2nd trimester vaccination (13-25 gestational weeks; GW), another study showed decreased effectiveness of 2nd trimester (<27 GW) vaccination. Only one study concerned antibody transfer in preterms and reported higher antibody levels after 2nd (n=37) than after 3rd (n=48) trimester vaccination. Aiming to contribute to setting optimal vaccine strategy of maternal pertussis vaccination in the Netherlands and elsewhere and particular for the most vulnerable group of preterms, we propose a study that compares pertussis antibody levels in preterms and terms after 2nd trimester maternal vaccination. We can compare these to data we have on 3rd trimester Tdap in terms. In addition to adequate antibody levels, success of 2nd trimester vaccination depends on acceptance of this strategy by pregnant women and professionals. Our primary endpoint is IgG anti-pertussis toxin (Pt) antibody concentration in preterms and terms at 2m of age, Pt is considered the most relevant antibody for protection against clinical pertussis. Secondary endpoints are e.g. pertussis specific antibody concentrations in preterms and terms in cord blood and in women at delivery. Determinants of acceptance of 2nd trimester maternal vaccination are also a secondary endpoint. Antibody concentrations will be assessed in serum, using a fluorescent bead-based multiplex immunoassay, with required blood volume of minimal 100µl. For the survey on acceptance, we aim to have 4 groups of 100 women each, i.e. women who are pregnant for the 1st time, women who already gave birth and in both groups women with and without a known increased risk of preterm delivery. For the immunogenicity part, we aim to have at least 60 preterms and 60 terms, as this is, according to experts, the minimum number to enable good comparisons. Pregnant women will be offered 2nd trimester pertussis vaccination. Both among acceptors and non-acceptors acceptance of 2nd trimester vaccination will be assessed. Women are first asked to participate in the acceptance part after the 1st antenatal visit to a midwife or obstetrician. They fill in a questionnaire to assess behavioral determinants and beliefs that underlie acceptance of 2nd trimester maternal vaccination. Only after this consent, women will be asked to participate in the immunogenicity part. Hereby, women will receive Tdap after they have the 20w standard anomaly ultrasound scan (20-24 GW). Vaccinated women will be followed until delivery. All preterms and a random selection of 60 terms, all of vaccinated mothers, will be followed until 2m of age, i.e. just before start of the NIP. By including both women in primary and secondary antenatal care, we aim to enrich our study population with women who are at increased risk for preterm delivery, as these women are usually seen by an obstetrician. Data from our study will determine whether 2nd trimester Tdap leads to sufficient Pt antibodiy concentration in terms and preterms compared to 3rd trimester vaccination. Furthermore, we will have knowledge about obstacles for acceptance and can tailor information for all pregnant women to overcome these. Finally, given that in near future besides pertussis other maternal vaccines are likely to become available for prevention of severe disease in newborns (RSV, GBS), in particular in preterms, this study generates essential knowledge for future vaccine policy of maternal vaccines.

<p>Datasets used for the manuscript:&nbsp;<em>Long-term wastewater monitoring of SARS-CoV-2 viral loads and variants at the major international passenger hub Amsterdam Schiphol Airport: a valuable addition to COVID-19 surveillance</em></p> <p><em>pandemic_daily_passenger_counts.tsv</em>: An overview of daily passenger arrival&nbsp;counts at Amsterdam Schiphol Airport per continent of origin during the study period 16-02-2020 - 04-09-2022</p> <p><em>pre-pandemic_daily_passenger_averages.tsv:&nbsp;</em>An overview of mean daily passenger arrival counts at Amsterdam Schiphol Airport in the pre-pandemic period 2017-2019.</p> <p><em>viral_load_data.tsv:&nbsp;</em>Flow-corrected viral load (# particles per 24h) in samples taken at the wastewater treatment plant of Amsterdam Schiphol Airport.</p> <p><em>wastewater_variant_frequencies.tsv:&nbsp;</em>SARS-CoV-2 lineage estimates in samples&nbsp;taken at the wastewater treatment plant of Amsterdam Schiphol Airport, analyzed using whole-genome tiled amplicon sequencing.</p>

In 2023 publiceert het RIVM een rapport dat verslag doet van een test van zelfbouwgeluidmeters. Deze dataset bevat de meetdata waarop de bevindingen van het rapport zijn gebaseerd. Het zijn gemiddelden: per dag, uur en 5 minuten. De betekenis van "NA" is dat er geen data is: die geluidmeter heeft in dat tijdvak niet gemeten.

Data is niet algemeen beschikbaar. CC-BY 4.0 licentie betreft de metadata niet de dataset De NECCST dataset omvat gegevens van 5700 vrouwen in Nederland die ooit hebben meegedaan aan de chlamydia screening implementatie tussen 2008 en 2011 en toen hebben aangegeven benaderd te kunnen worden voor vervolgonderzoek naar seksueel overdraagbare aandoeningen. Dat is in 2015 gebeurd in het kader van de Nederlandse chlamydia cohort studie (NECCST). Door middel van een informed consent kregen vrouwen toegang tot een online vragenlijst met vragen over demografie, chlamydia historie, zwangerschappen, lange termijn complicaties van chlamydia zoals chronische buikpijn, buitenbaarmoederlijke zwangerschappen, infertiliteit, anticonceptie en seksueel risico gedrag. De vragen opnieuw gesteld in 2017 en 2019. The NECCST dataset contains information of 5700 females in the Netherlands who participated in the Chlamydia Screening Implementation project between 2008 and 2011. All women that agreed to be asked for follow-up research were invited to participate in the Netherlands Chlamydia Cohort Study in 2015. After informed consent women answered questions in an online survey on demographics, history of chlamydia, pregnancies, long term complications of chlamydia like pelvic inflammatory disease (PID), ectopic pregnancies, tubal factor infertility, anticonception and sexual risk behavior. Other questionnaire rounds took place in 2017 and 2019.

Genexpressie in rattenlong na inhalatie van EHC-93 fijnstof

The dataset “Impact of HIV strategies for MSM” contains data obtained from an agent-based model. The model follows the sexual life of 20,000 men who have sex with men (MSM) in the Netherlands. Via sexual contacts, men may get infected with HIV or N. Gonorrhoeae (NG). The model simulates sexual behaviour, demography, and the course of HIV or NG infection (for those who have been infected). The data from the model are therefore data of “fictitious” (simulated) individuals, not of real individuals. The course of HIV infection was modelled using data from the national database of HIV-positive individuals in the Netherlands (Source: Stichting HIV Monitoring). Parameters relating to sexual behaviour were obtained from data from the Amsterdam Cohort Study and the Network Study among MSM in Amsterdam. The model was calibrated to data on annual HIV diagnoses in 2008-2014 (from Stichting HIV Monitoring) and gonorrhoea positivity in 2009-2014 (data obtained from the National Database of STI Clinics in the Netherlands (SOAP)). Model outcomes include the annual numbers of MSM getting infected with HIV; HIV-positive MSM getting diagnosed, entering care, or starting treatment; MSM developing AIDS; MSM getting infected with NG; MSM treated for gonorrhoea; HIV tests, NG tests, etc. With the model, we calculated these numbers for the years 2018-2027, for the situation with the current testing rates and without PrEP. Subsequently we calculated these numbers with increased HIV/STI testing: a small, a moderate, and a high increase in testing among all MSM or only among MSM in specific subgroups of MSM. Finally, the calculations were repeated accounting for a nationwide PrEP programme for MSM at high risk to acquire HIV.

Genexpressie in borstklieren van muizen na vroege blootstelling aan dieten met verschillende soorten vetzuren

Genexpressie in testes van twee muismodellen na blootstelling aan benzo(a)pyreen; normale WT en DNA repair deficiente Xpc-/- muizen.

Van 1 januari 2019 tot 1 juli 2021 is kwalitatieve en kwantitatieve data verzameld in een onderzoeksproject getiteld “An innovative network-based approach to study and enable well-inFormed dEcision-Making among TurkIsh- and MoroccaN-Dutch women regarding cervIcal caNcer scrEening: the FEMININE study”. Hierbij zijn drie verschillende onderzoeken (Fase I, II en III) uitgevoerd. In Fase I is er kwalitatieve data verzameld en zijn er zes focusgroepen uitgevoerd, drie onder Turks- en drie onder Marokkaans-Nederlandse vrouwen. Het doel was om te achterhalen hoe en waarom Turks- en Marokkaans-Nederlandse vrouwen van 30-60 jaar de keuze maken om wel of niet deel te nemen aan het bevolkingsonderzoek baarmoederhalskanker. In Fase II is er kwantitatieve data verzameld middels een vragenlijst. Er is informatie verzameld bij Turks- en Marokkaans-Nederlandse vrouwen van 30-60 jaar over demografische factoren, eerdere deelname aan het bevolkingsonderzoek baarmoederhalskanker, het maken van de keuze om wel of niet mee te doen aan het bevolkingsonderzoek (‘decisional conflict’), redenen om wel of niet mee te doen aan het bevolkingsonderzoek, kennis over het bevolkingsonderzoek en de zelfafnameset, attitude aangaande het bevolkingsonderzoek en de zelfafnameset, de intentie om wel of niet mee te doen aan het bevolkingsonderzoek en de voorkeur om dit te doen middels het uitstrijkje of de zelfafnameset. Het doel was om te bepalen in hoeverre Turks- en Marokkaans-Nederlandse vrouwen een geïnformeerde beslissing maken en welke determinanten daaraan ten grondslag liggen. In Fase III is er een gerandomiseerde interventiestudie uitgevoerd middels een online vragenlijst. Deelnemers werden gerandomiseerd over een controlegroep (kregen alleen de huidige informatiefolder te zien) of een interventiegroep (kregen een combinatie van de huidige informatiefolder en een cultuur sensitief filmpje in de door hen gekozen taal te zien). Het doel van dit onderzoek was om te evalueren of en in hoeverre het op basis van Fase II ontwikkeld filmpje in staat was om vrouwen te helpen bij het maken van een geïnformeerde beslissing t.a.v. het bevolkingsonderzoek baarmoederhalskanker. Hierbij is er informatie verzameld bij Turks- en Marokkaans-Nederlandse vrouwen van 30-60 jaar over demografische factoren, eerdere deelname aan het bevolkingsonderzoek baarmoederhalskanker, kennis over het bevolkingsonderzoek en de zelfafnameset, attitude aangaande het bevolkingsonderzoek en de zelfafnameset, de intentie om wel of niet mee te doen aan het bevolkingsonderzoek middels een uitstrijkje dan wel de zelfafnameset en de grootte van het sociale netwerk. Tot slot werden deelnemers gevraagd om de vragenlijst ook door te sturen naar vrouwen in hun sociale netwerk.

Genexpressie in keratinocyten na blootstelling aan sensibiliserende of irriterende stoffen